Screening for anti-inflammatory activity of extract and fractions of Morinda lucida stem bark and detection of bioactive secondary metabolite

Morinda lucida Benth (Rubiaceae) is a versatile plant used in traditional medicine for the treatment of variety ailments and claims its efficacy are particularly remarkable infections immuno-inflammatory disorders. This study evaluates anti-inflammatory properties methanolic stem bark extract fractions M. also identifies phytochemicals responsible activity. The crude was subjected to liquid- liquid partitioning successively with n- hexane, ethylacetate, butanol water. High performance chromatography (HPLC) four Vacuum Liquid Chromatography fraction (VLC) promising evaluated. effect on egg albumen induced rat paw oedema were Anti-inflammatory activity further screened using xylene induce ear models human red blood cell membrane stabilization test. Ulcerogenic test normal stomach mucosa result showed that maximally inhibited at 400 mg/kg (70%) 200mg/kg (67.5%) after 180 minutes compared positive control, ibuprofen (20mg/kg) 100% inhibition minutes. produced by 100 µg/ear Butanol (BF) 56.67 % greater than 200 (38.89 %). HPLC analysis revealed following phytocompounds; Cytreo- a-pyrone, Cytosporin- J Waol A. negative doses when compare indomethacin (positive control) dose 50 mg/kg. Human assay BF-VLC 2 (Dichloromethane: methanol (8:2) VLC fraction) exhibited concentration dependent heat-induced haemolysis while other non- standard, ibuprofen. These findings suggest possess phytocompounds which justify use ethno-medicine.